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mouse cerebral cortex endothelioma cells  (ATCC)


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    Structured Review

    ATCC mouse cerebral cortex endothelioma cells
    Mouse Cerebral Cortex Endothelioma Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1913 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+cerebral+cortex+endothelioma+cells/bEnd%2E3/pmc11296219-604-14-20
    Average 99 stars, based on 1913 article reviews
    mouse cerebral cortex endothelioma cells - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Modification:

    Article Title: Isoform-specific modification of apolipoprotein E by 4-hydroxynonenal: protective role of apolipoprotein E3 against oxidative species.
    Article Snippet: doi:10.1111/febs.16729 High levels of 4-hydroxynonenal (HNE), arising from lipid peroxidation, and HNE-modified proteins have been identified in postmortem brains of ageing and Alzheimer’s disease (AD) patients.. The goal of this study is to understand the effect of HNE modification on the structure and function of recombinant apolipoprotein E3 (apoE3) and apolipoprotein E4 (apoE4), which play a critical role in brain cholesterol homeostasis.. The two isoforms differ in a single amino acid at position 112: Cys in apoE3 and Arg in apoE4.

    Article Title: Isoform-specific modification of apolipoprotein E by 4-hydroxynonenal: protective role of apolipoprotein E3 against oxidative species
    Article Snippet: In parallel, the presence of sLDLr in each incubation was confirmed by probing with an anti- c-myc antibody (9E10; 1 : 1000 dilution; Abcam, Waltham, MA, USA) and goat anti-mouse HRP antibody (1 : 5000 dilution; Abcam, Waltham, MA, USA). .. The cellular uptake of DiI-labelled unmodified and modified apoE3/rHDL and apoE4/rHDL was visualized in mouse cerebral cortex endothelioma cells (bEnd.3; American Type Culture Collection, Manassas, VA, USA) by fluorescence microscopy. .. The cells (3.5–5.0 × 10 5 cells) were grown in sixwell plates (Corning, Corning, NY, USA) in complete DMEM until they reached ~ 80% confluency, after which they were grown in 10% lipoprotein deficient serum (LPDS) in DMEM for an additional 24 h prior to addition of samples.

    Article Title: Isoform-specific modification of apolipoprotein E by 4-hydroxynonenal: protective role of apolipoprotein E3 against oxidative species
    Article Snippet: In parallel, the presence of sLDLr in each incubation was confirmed by probing with an anti- c-myc antibody (9E10; 1 : 1000 dilution; Abcam, Waltham, MA, USA) and goat anti-mouse HRP antibody (1 : 5000 dilution; Abcam, Waltham, MA, USA). .. Fluorescence microscopy The cellular uptake of DiI-labelled unmodified and modified apoE3/rHDL and apoE4/rHDL was visualized in mouse cerebral cortex endothelioma cells (bEnd.3; American Type Culture Collection, Manassas, VA, USA) by fluorescence microscopy. .. The cells (3.5–5.0 × 10 5 cells) were grown in sixwell plates (Corning, Corning, NY, USA) in complete DMEM until they reached ~ 80% confluency, after which they were grown in 10% lipoprotein deficient serum (LPDS) in DMEM for an additional 24 h prior to addition of samples.

    Fluorescence:

    Article Title: Isoform-specific modification of apolipoprotein E by 4-hydroxynonenal: protective role of apolipoprotein E3 against oxidative species.
    Article Snippet: doi:10.1111/febs.16729 High levels of 4-hydroxynonenal (HNE), arising from lipid peroxidation, and HNE-modified proteins have been identified in postmortem brains of ageing and Alzheimer’s disease (AD) patients.. The goal of this study is to understand the effect of HNE modification on the structure and function of recombinant apolipoprotein E3 (apoE3) and apolipoprotein E4 (apoE4), which play a critical role in brain cholesterol homeostasis.. The two isoforms differ in a single amino acid at position 112: Cys in apoE3 and Arg in apoE4.

    Article Title: Isoform-specific modification of apolipoprotein E by 4-hydroxynonenal: protective role of apolipoprotein E3 against oxidative species
    Article Snippet: In parallel, the presence of sLDLr in each incubation was confirmed by probing with an anti- c-myc antibody (9E10; 1 : 1000 dilution; Abcam, Waltham, MA, USA) and goat anti-mouse HRP antibody (1 : 5000 dilution; Abcam, Waltham, MA, USA). .. The cellular uptake of DiI-labelled unmodified and modified apoE3/rHDL and apoE4/rHDL was visualized in mouse cerebral cortex endothelioma cells (bEnd.3; American Type Culture Collection, Manassas, VA, USA) by fluorescence microscopy. .. The cells (3.5–5.0 × 10 5 cells) were grown in sixwell plates (Corning, Corning, NY, USA) in complete DMEM until they reached ~ 80% confluency, after which they were grown in 10% lipoprotein deficient serum (LPDS) in DMEM for an additional 24 h prior to addition of samples.

    Article Title: Isoform-specific modification of apolipoprotein E by 4-hydroxynonenal: protective role of apolipoprotein E3 against oxidative species
    Article Snippet: In parallel, the presence of sLDLr in each incubation was confirmed by probing with an anti- c-myc antibody (9E10; 1 : 1000 dilution; Abcam, Waltham, MA, USA) and goat anti-mouse HRP antibody (1 : 5000 dilution; Abcam, Waltham, MA, USA). .. Fluorescence microscopy The cellular uptake of DiI-labelled unmodified and modified apoE3/rHDL and apoE4/rHDL was visualized in mouse cerebral cortex endothelioma cells (bEnd.3; American Type Culture Collection, Manassas, VA, USA) by fluorescence microscopy. .. The cells (3.5–5.0 × 10 5 cells) were grown in sixwell plates (Corning, Corning, NY, USA) in complete DMEM until they reached ~ 80% confluency, after which they were grown in 10% lipoprotein deficient serum (LPDS) in DMEM for an additional 24 h prior to addition of samples.

    Microscopy:

    Article Title: Isoform-specific modification of apolipoprotein E by 4-hydroxynonenal: protective role of apolipoprotein E3 against oxidative species.
    Article Snippet: doi:10.1111/febs.16729 High levels of 4-hydroxynonenal (HNE), arising from lipid peroxidation, and HNE-modified proteins have been identified in postmortem brains of ageing and Alzheimer’s disease (AD) patients.. The goal of this study is to understand the effect of HNE modification on the structure and function of recombinant apolipoprotein E3 (apoE3) and apolipoprotein E4 (apoE4), which play a critical role in brain cholesterol homeostasis.. The two isoforms differ in a single amino acid at position 112: Cys in apoE3 and Arg in apoE4.

    Article Title: Isoform-specific modification of apolipoprotein E by 4-hydroxynonenal: protective role of apolipoprotein E3 against oxidative species
    Article Snippet: In parallel, the presence of sLDLr in each incubation was confirmed by probing with an anti- c-myc antibody (9E10; 1 : 1000 dilution; Abcam, Waltham, MA, USA) and goat anti-mouse HRP antibody (1 : 5000 dilution; Abcam, Waltham, MA, USA). .. The cellular uptake of DiI-labelled unmodified and modified apoE3/rHDL and apoE4/rHDL was visualized in mouse cerebral cortex endothelioma cells (bEnd.3; American Type Culture Collection, Manassas, VA, USA) by fluorescence microscopy. .. The cells (3.5–5.0 × 10 5 cells) were grown in sixwell plates (Corning, Corning, NY, USA) in complete DMEM until they reached ~ 80% confluency, after which they were grown in 10% lipoprotein deficient serum (LPDS) in DMEM for an additional 24 h prior to addition of samples.

    Article Title: Isoform-specific modification of apolipoprotein E by 4-hydroxynonenal: protective role of apolipoprotein E3 against oxidative species
    Article Snippet: In parallel, the presence of sLDLr in each incubation was confirmed by probing with an anti- c-myc antibody (9E10; 1 : 1000 dilution; Abcam, Waltham, MA, USA) and goat anti-mouse HRP antibody (1 : 5000 dilution; Abcam, Waltham, MA, USA). .. Fluorescence microscopy The cellular uptake of DiI-labelled unmodified and modified apoE3/rHDL and apoE4/rHDL was visualized in mouse cerebral cortex endothelioma cells (bEnd.3; American Type Culture Collection, Manassas, VA, USA) by fluorescence microscopy. .. The cells (3.5–5.0 × 10 5 cells) were grown in sixwell plates (Corning, Corning, NY, USA) in complete DMEM until they reached ~ 80% confluency, after which they were grown in 10% lipoprotein deficient serum (LPDS) in DMEM for an additional 24 h prior to addition of samples.



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    ATCC mouse cerebral cortex endothelioma cells
    Mouse Cerebral Cortex Endothelioma Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+cerebral+cortex+endothelioma+cells/bEnd%2E3/pmc11296219-604-14-20
    Average 99 stars, based on 1 article reviews
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